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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Anti-STX1A Antibody

HPA069176
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTX1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STX1A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6804
  • Target name
    STX1A
  • Target description
    syntaxin 1A
  • Target synonyms
    HPC-1, P35-1, STX1, SYN1A, syntaxin-1A, neuron-specific antigen HPC-1, syntaxin 1A (brain)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16623
  • Protein Name
    Syntaxin-1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa.
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