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ProductsBack/Goat anti-STX1A / STX1B

Goat anti-STX1A / STX1B

EB06881
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSTX1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-STX1A / STX1B Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6804
  • Target name
    STX1A
  • Target description
    syntaxin 1A
  • Target synonyms
    HPC-1; neuron-specific antigen HPC-1; P35-1; STX1; SYN1A; syntaxin 1A (brain); syntaxin-1A
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004594.1; NP_001159375.1; NP_443106.1. GeneIDs all Nonhuman: 20907 (mouse); 116470 (rat);. Purification: Antigen affinity purified. Names and symbols: STX1A; syntaxin 1A (brain) ; HPC-1; STX1; p35-1; SYN1A; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; neuron-specific antigen HPC-1; syntaxin-1A; STX1B; syntaxin 1B; STX1B1; STX1B2; FP17469; OTTHUMP00000163224; syntaxin 1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa.
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