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Western Blot analysis of HT29 MCF7 cells using Syntaxin 1 Polyclonal Antibody
Western Blot analysis of HT29 MCF7 cells using Syntaxin 1 Polyclonal Antibody
Western Blot analysis of HT29 MCF7 cells using Syntaxin 1 Polyclonal Antibody

STX1A Antibody

CSB-PA004208
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetSTX1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    STX1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6804
  • Target name
    STX1A
  • Target description
    syntaxin 1A
  • Target synonyms
    HPC-1; neuron-specific antigen HPC-1; P35-1; STX1; SYN1A; syntaxin 1A (brain); syntaxin-1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16623
  • Protein Name
    Syntaxin-1A
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa.
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