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Syntaxin 1A Antibody (YA2001, Rabbit)

HY-P82256
MedChem Express
TargetSTX1A
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Syntaxin 1A Antibody (YA2001, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6804
  • Target name
    STX1A
  • Target description
    syntaxin 1A
  • Target synonyms
    HPC-1, P35-1, STX1, SYN1A, syntaxin-1A, neuron-specific antigen HPC-1, syntaxin 1A (brain)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Syntaxin 1A Antibody (YA2001) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2001), targeting Syntaxin 1A, with a predicted molecular weight of 33 kDa (observed band size: 33 kDa). Syntaxin 1A Antibody (YA2001) can be used for WB, IP, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa.
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