
Figure 1. Western blot analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12 antigen affinity purified polyclonal antibody (Catalog # A00583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUZ12 at approximately 90KD. The expected band size for SUZ12 is at 83KD.
Anti-SUZ12 Antibody Picoband(r)
A00583-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSUZ12
Overview
- SupplierBoster Bio
- Product NameAnti-SUZ12 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID23512
- Target nameSUZ12
- Target descriptionSUZ12 polycomb repressive complex 2 subunit
- Target synonymschET 9 protein; CHET9; chromatin precipitated E2F target 9 protein; IMMAS; JJAZ1; joined to JAZF1 protein; polycomb protein SUZ12; suppressor of zeste 12 protein homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ15022
- Protein NamePolycomb protein SUZ12
- Scientific DescriptionBoster Bio Anti-SUZ12 Antibody Picoband® catalog # A00583-1. Tested in ELISA, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203