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Western Blot Positive WB detected in: Rat kidney tissue, Rat liver tissue All lanes: SUZ12 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa
Western Blot Positive WB detected in: Rat kidney tissue, Rat liver tissue All lanes: SUZ12 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa
Western Blot Positive WB detected in: Rat kidney tissue, Rat liver tissue All lanes: SUZ12 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa

SUZ12 Antibody

CSB-PA621859LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSUZ12
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Overview

  • Supplier
    Cusabio
  • Product Name
    SUZ12 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23512
  • Target name
    SUZ12
  • Target description
    SUZ12 polycomb repressive complex 2 subunit
  • Target synonyms
    chET 9 protein; CHET9; chromatin precipitated E2F target 9 protein; IMMAS; JJAZ1; joined to JAZF1 protein; polycomb protein SUZ12; suppressor of zeste 12 protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15022
  • Protein Name
    Polycomb protein SUZ12
  • Scientific Description
    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates Lys-9 (H3K9me) and Lys-27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12 antigen affinity purified polyclonal antibody (Catalog # A00583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUZ12 at approximately 90KD. The expected band size for SUZ12 is at 83KD.
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