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SUZ12 Antibody

LS-C409335
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSUZ12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUZ12 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 83kDa, while the observed MW by Western blot was 83kDa.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), IP (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 83kDa, while the observed MW by Western blot was 83kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23512
  • Target name
    SUZ12
  • Target description
    SUZ12 polycomb repressive complex 2 subunit
  • Target synonyms
    chET 9 protein; CHET9; chromatin precipitated E2F target 9 protein; IMMAS; JJAZ1; joined to JAZF1 protein; polycomb protein SUZ12; suppressor of zeste 12 protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12 antigen affinity purified polyclonal antibody (Catalog # A00583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUZ12 at approximately 90KD. The expected band size for SUZ12 is at 83KD.
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