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Immunoprecipitation of SUZ12 protein from 293T whole cell extracts using 5 μg of SUZ12 antibody (GTX130179). Western blot analysis was performed using SUZ12 antibody (GTX130179) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of SUZ12 protein from 293T whole cell extracts using 5 μg of SUZ12 antibody (GTX130179). Western blot analysis was performed using SUZ12 antibody (GTX130179) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of SUZ12 protein from 293T whole cell extracts using 5 μg of SUZ12 antibody (GTX130179). Western blot analysis was performed using SUZ12 antibody (GTX130179) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SUZ12 antibody

GTX130179
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSUZ12
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Overview

  • Supplier
    GeneTex
  • Product Name
    SUZ12 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23512
  • Target name
    SUZ12
  • Target description
    SUZ12 polycomb repressive complex 2 subunit
  • Target synonyms
    CHET9, IMMAS, JJAZ1, polycomb protein SUZ12, chET 9 protein, chromatin precipitated E2F target 9 protein, joined to JAZF1 protein, suppressor of zeste 12 protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15022
  • Protein Name
    Polycomb protein SUZ12
  • Scientific Description
    This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. The specific function of this gene has not yet been determined. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12 antigen affinity purified polyclonal antibody (Catalog # A00583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUZ12 at approximately 90KD. The expected band size for SUZ12 is at 83KD.
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