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Immunohistochemical staining of human skin shows strong membranous positivity in basal cells.
Immunohistochemical staining of human skin shows strong membranous positivity in basal cells.
Immunohistochemical staining of human skin shows strong membranous positivity in basal cells.

Anti-TACR1 Antibody

HPA074573
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTACR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TACR1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6869
  • Target name
    TACR1
  • Target description
    tachykinin receptor 1
  • Target synonyms
    NK1R, NKIR, SPR, TAC1R, substance-P receptor, NK-1 receptor, NK-1R, neurokinin receptor 1, tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25103
  • Protein Name
    Substance-P receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa.
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