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Goat anti-TACR1

EB07771
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetTACR1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TACR1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Expression of the protein seen in the plasma membrane and nucleus of Neuro2a cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6869
  • Target name
    TACR1
  • Target description
    tachykinin receptor 1
  • Target synonyms
    neurokinin receptor 1; NK-1 receptor; NK1R; NK-1R; NKIR; SPR; substance-P receptor; TAC1R; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001049.1*; NP_056542.1*. GeneIDs all Nonhuman: 21336 (mouse); 24807 (rat);. Purification: Antigen affinity purified. Names and symbols: TACR1; tachykinin receptor 1; NK1R; neurokinin 1 receptor; NKIR; SPR; TAC1R ; NK-1 receptor; Tachykinin receptor 1 (substance P receptor,neurokinin-1 receptor); neurokinin 1 receptor; tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
  • Reactivity Supplier
    Human, Mouse, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa.
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