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Neurokinin 1 Receptor antibody [HL3194]

GTX640833
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetTACR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Neurokinin 1 Receptor antibody [HL3194]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL3194
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6869
  • Target name
    TACR1
  • Target description
    tachykinin receptor 1
  • Target synonyms
    neurokinin receptor 1; NK-1 receptor; NK1R; NK-1R; NKIR; SPR; substance-P receptor; TAC1R; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25103
  • Protein Name
    Substance-P receptor
  • Scientific Description
    This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa.
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