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Western Blot analysis of MOUSE-BRAIN COLO cells using NK-1R Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN COLO cells using NK-1R Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN COLO cells using NK-1R Polyclonal Antibody

TACR1 Antibody

CSB-PA050201
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTACR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TACR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6869
  • Target name
    TACR1
  • Target description
    tachykinin receptor 1
  • Target synonyms
    NK1R, NKIR, SPR, TAC1R, substance-P receptor, NK-1 receptor, NK-1R, neurokinin receptor 1, tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25103
  • Protein Name
    Substance-P receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa.
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