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Anti-Tau [PC1C6]

Ab01120-2.0
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMAPT
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Tau [PC1C6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    In the original study, this antibody was used in immunoblot assays and competitive ELISA to show that tau was more abundant in bovine white matter extracts and microtubules than in extracts and microtubules from an enriched gray matter region of the brain (Binder et al., 1985). Example of functional studies in which this antibody could be utilised include those conducted by Kosik et al. (PNAS 1986) to confirm that tau is a major antigenic component of paired helical filaments in Alzheimer disease, by Bramblett et al. (Neuron 1993) to demonstrate that abnormal tau phosphorylation at Ser396 in Alzheimers disease recapitulates development and contributes to reduced microtubule binding, by Stamer et al. (J Cell Biol. 2002) to suggests a linkage between tau and amyloid precursor protein (APP) trafficking, and by Strang et al. (JBC 2018) to provide novel insights in the molecular mechanisms of tau aggregation.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PC1C6
  • Gene ID4137
  • Target name
    MAPT
  • Target description
    microtubule associated protein tau
  • Target synonyms
    DDPAC; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; microtubule-associated protein tau; MSTD; MTBT1; MTBT2; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; tau-40
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP10636
  • Protein Name
    Microtubule-associated protein tau
  • Reactivity Supplier
    Human, Rat, Bovine
  • Reactivity Supplier Note
    This antibody was raised by immunising BALB/c mouse with purified denatured bovine microtubule associated proteins.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tau/MAPT antigen affinity purified polyclonal antibody (Catalog # A00097-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tau/MAPT at approximately 50-70 kDa. The expected band size for Tau/MAPT is at 79 kDa.
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