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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tTau protein Polyclonal Antibody, Unconjugated (bs-0157R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tTau protein Polyclonal Antibody, Unconjugated (bs-0157R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tTau protein Polyclonal Antibody, Unconjugated (bs-0157R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.

Tau protein Polyclonal Antibody

Research Use Only
BS-0157R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetMAPT
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Tau protein Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4137
  • Target name
    MAPT
  • Target description
    microtubule associated protein tau
  • Target synonyms
    DDPAC; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; microtubule-associated protein tau; MSTD; MTBT1; MTBT2; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; tau-40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10636
  • Protein Name
    Microtubule-associated protein tau
  • Reactivity
    Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/beta-Catenin Signaling. Li YH et al., 2021 Feb, Chin J Integr Med
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  • Indole Compound NC009-1 Augments APOE and TRKA in Alzheimers Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. Chen YC et al., 2019, J Alzheimers Dis
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  • Synergistic effects of particulate matter (PM2.5) and sulfur dioxide (SO2) on neurodegeneration via the microRNA-mediated regulation of tau phosphorylation. Ku T et al., 2017 Jan 1, Toxicol Res (Camb)
    Read more
  • Combinated transplantation of neural stem cells and collagen type I promote functional recovery after cerebral ischemia in rats. Yu H et al., 2010 May, Anat Rec (Hoboken)
    Read more