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ProductsBack/MAPT / Tau Antibody (phospho-Ser199, clone 17D3.G7)

MAPT / Tau Antibody (phospho-Ser199, clone 17D3.G7)

LS-C342879
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetMAPT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAPT / Tau Antibody (phospho-Ser199, clone 17D3.G7)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:1000 - 1:2000), IP, WB (2 - 5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17D3.G7
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4137
  • Target name
    MAPT
  • Target description
    microtubule associated protein tau
  • Target synonyms
    DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40, microtubule-associated protein tau, G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, Tau-derived paired helical filament hexapeptide, neurofibrillary tangle protein, paired helical filament-tau, protein phosphatase 1, regulatory subunit 103
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tau/MAPT antigen affinity purified polyclonal antibody (Catalog # A00097-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tau/MAPT at approximately 50-70 kDa. The expected band size for Tau/MAPT is at 79 kDa.
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