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Anti-TGFalpha [tAb2]

AB00614-2.0-BT
Absolute Antibody
ApplicationsDot Blot, ELISA, Other Application
Product group Antibodies
TargetTGFA
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TGFalpha [tAb2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Human transforming growth factora (TGFa) consists of 50 amino acid residues with three disulphide bridges. It belongs to the family of epidermal growth factor related peptides and binds to EGFreceptor. It is involved in cell proliferation in healthy cells and is found in various cancer cells. Transfection of Fibroblasts with the TGFa gene induces tumor formation.
  • Applications
    Dot Blot, ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    tAb2
  • Gene ID7039
  • Target name
    TGFA
  • Target description
    transforming growth factor alpha
  • Target synonyms
    protransforming growth factor alpha; TFGA; TGF-alpha
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP01135
  • Protein Name
    Protransforming growth factor alpha
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
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