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Western Blot analysis of COS7 cells using TGFalpha Polyclonal Antibody
Western Blot analysis of COS7 cells using TGFalpha Polyclonal Antibody
Western Blot analysis of COS7 cells using TGFalpha Polyclonal Antibody

TGFA Antibody

CSB-PA004275
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetTGFA
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGFA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7039
  • Target name
    TGFA
  • Target description
    transforming growth factor alpha
  • Target synonyms
    protransforming growth factor alpha; TFGA; TGF-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01135
  • Protein Name
    Protransforming growth factor alpha
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
Product group Antibodies
References
Boster Bio
Anti-TGF alpha Monoclonal AntibodyM01779-1
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TargetTGFA
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Hela cells probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) for 30 minutes followed by incubation with a conjugated secondary (FITC conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
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TargetTGFA
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TargetTGFA
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ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetTGFA
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TargetTGFA
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TargetTGFA
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Product group Antibodies
Atlas Antibodies
Anti-TGFA AntibodyHPA042297
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TargetTGFA
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LifeSpan BioSciences
TGFA / TGF Alpha AntibodyLS-C401080
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TargetTGFA
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