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TGFA Polyclonal Antibody

CAC14099
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTGFA
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Overview

  • Supplier
    Biomatik
  • Product Name
    TGFA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7039
  • Target name
    TGFA
  • Target description
    transforming growth factor alpha
  • Target synonyms
    protransforming growth factor alpha; TFGA; TGF-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01135
  • Protein Name
    Protransforming growth factor alpha
  • Scientific Description
    The TGFA Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
Product group Antibodies
References
Boster Bio
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Hela cells probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) for 30 minutes followed by incubation with a conjugated secondary (FITC conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
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