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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-TICAM1 Antibody

HPA042460
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTICAM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TICAM1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID148022
  • Target name
    TICAM1
  • Target description
    TIR domain containing adaptor molecule 1
  • Target synonyms
    IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF, TIR domain-containing adapter molecule 1, TIR domain containing adaptor inducing interferon-beta, TIR domain-containing adapter protein inducing IFN-beta, proline-rich, vinculin and TIR domain-containing protein B, putative NF-kappa-B-activating protein 502H, toll like receptor adaptor molecule 1, toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IUC6
  • Protein Name
    TIR domain-containing adapter molecule 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRIF/TICAM1 using anti-TRIF/TICAM1 antibody (A01872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF/TICAM1 antigen affinity purified polyclonal antibody (Catalog # A01872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIF/TICAM1 at approximately 110KD. The expected band size for TRIF/TICAM1 is at 76KD.
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