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ProductsBack/TRIF antibody [C3], C-term

TRIF antibody [C3], C-term

GTX104744
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetTICAM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRIF antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.01 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID148022
  • Target name
    TICAM1
  • Target description
    toll like receptor adaptor molecule 1
  • Target synonyms
    IIAE6; MyD88-3; proline-rich, vinculin and TIR domain-containing protein B; PRVTIRB; putative NF-kappa-B-activating protein 502H; TICAM-1; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adapter protein inducing IFN-beta; toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; TRIF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IUC6
  • Protein Name
    TIR domain-containing adapter molecule 1
  • Scientific Description
    TRIF, or TICAM1, is a Toll/IL1R (MIM 147810) (TIR) domain-containing adaptor molecule, like MYD88 (MIM 602170) and TIRAP (MIM 606252), that induces interferon-beta (IFNB; MIM 147640), specifically interacts with TLR3 (MIM 603029), and activates nuclear factor kappa-B (NFKB; see MIM 164011).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages. Mlcochova P et al., 2020 Mar 24, Cell Rep
    Read more

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Figure 1. Western blot analysis of TRIF/TICAM1 using anti-TRIF/TICAM1 antibody (A01872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF/TICAM1 antigen affinity purified polyclonal antibody (Catalog # A01872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIF/TICAM1 at approximately 110KD. The expected band size for TRIF/TICAM1 is at 76KD.
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