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Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

TICAM1 Antibody

CSB-PA544544
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTICAM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TICAM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID148022
  • Target name
    TICAM1
  • Target description
    TIR domain containing adaptor molecule 1
  • Target synonyms
    IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF, TIR domain-containing adapter molecule 1, TIR domain containing adaptor inducing interferon-beta, TIR domain-containing adapter protein inducing IFN-beta, proline-rich, vinculin and TIR domain-containing protein B, putative NF-kappa-B-activating protein 502H, toll like receptor adaptor molecule 1, toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IUC6
  • Protein Name
    TIR domain-containing adapter molecule 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIF/TICAM1 using anti-TRIF/TICAM1 antibody (A01872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF/TICAM1 antigen affinity purified polyclonal antibody (Catalog # A01872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIF/TICAM1 at approximately 110KD. The expected band size for TRIF/TICAM1 is at 76KD.
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