Bio-Connect
Anti-TNF Antibody

Anti-TNF Antibody

A101582
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TNF Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to TNF.
  • UNSPSC
    12352203

Related products

Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTNF
  • SizePrice
Anti-human TNF-alpha mAb (MT25C5), unconjugated
Product group Antibodies
Mabtech
ApplicationsELISA, ELISpot Assay
TargetTNF
  • SizePrice
Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
TargetTNF
  • SizePrice
Product group Antibodies
MedChem Express
TargetTNF
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTNF
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
TargetTNF
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ELISA
TargetTNF
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Other Application
TargetTNF
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNF
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice