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Anti-human TNF-alpha mAb (MT25C5), unconjugated

Anti-human TNF-alpha mAb (MT25C5), unconjugated

3512-3
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetTNF
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human TNF-alpha mAb (MT25C5), unconjugated
  • Delivery Days Customer
    5
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT25C5
  • Conjugate
    Unconjugated
  • Gene ID7124
  • Target name
    TNF
  • Target description
    tumor necrosis factor
  • Target synonyms
    DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human TNF-alpha in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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