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Anti-TNF alpha [cA2 (Infliximab)]

Ab00146-10.0
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Other Application
Product group Antibodies
TargetTNF
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TNF alpha [cA2 (Infliximab)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used to detect and neutralize human TNF alpha. Unlike Adalimumab (Ab00718), Infliximab does not bind to mouse TNF alpha (Arnoult 2017, PMID: 28584008).
  • Applications
    Flow Cytometry, ELISA, Other Application
  • Applications Supplier
    ELISA; in vivo; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    cA2 (Infliximab)
  • Gene ID7124
  • Target name
    TNF
  • Target description
    tumor necrosis factor
  • Target synonyms
    APC1 protein; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01375
  • Protein Name
    Tumor necrosis factor
  • Scientific Description
    NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Purified recombinant human TNF (rTNF).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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