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Western Blot analysis of THP-1 mouse heart cells using TREM-1 Polyclonal Antibody
Western Blot analysis of THP-1 mouse heart cells using TREM-1 Polyclonal Antibody
Western Blot analysis of THP-1 mouse heart cells using TREM-1 Polyclonal Antibody

TREM1 Antibody

CSB-PA004863
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTREM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TREM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54210
  • Target name
    TREM1
  • Target description
    triggering receptor expressed on myeloid cells 1
  • Target synonyms
    CD354; TREM-1; triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP99
  • Protein Name
    Triggering receptor expressed on myeloid cells 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Hcclm3 cell lysates; Lane 2: K562 cell lysates probed with TREM1 Polyclonal Antibody, Unconjugated (bs-10306R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TREM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
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