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CD354 Antibody (YA4511, Mouse)

HY-P84814
MedChem Express
TargetTREM1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CD354 Antibody (YA4511, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID54210
  • Target name
    TREM1
  • Target description
    triggering receptor expressed on myeloid cells 1
  • Target synonyms
    CD354, TREM-1, triggering receptor expressed on myeloid cells 1, triggering receptor expressed on monocytes 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    CD354 Antibody (YA4511) is a mouse-derived and non-conjugated IgG1 antibody, targeting to CD354. It can be applicated for WB, FC, ELISA assays, in the background of human, rat, monkey.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TREM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
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