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Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in Leydig cells.

Anti-TSTA3 Antibody

HPA023361
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGFUS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TSTA3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7264
  • Target name
    GFUS
  • Target description
    GDP-L-fucose synthase
  • Target synonyms
    FX, P35B, SDR4E1, TSTA3, GDP-L-fucose synthase, 3-5 epimerase/4-reductase, GDP-4-keto-6-deoxy-D-mannose epimerase-reductase, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, red cell NADP(H)-binding protein, short chain dehydrogenase/reductase family 4E, member 1, testis tissue sperm-binding protein Li 45a, tissue specific transplantation antigen 3, tissue specific transplantation antigen P35B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13630
  • Protein Name
    GDP-L-fucose synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TSTA3/GFUS using anti-TSTA3/GFUS antibody (A09921-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSTA3/GFUS antigen affinity purified polyclonal antibody (Catalog # A09921-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSTA3/GFUS at approximately 39 kDa. The expected band size for TSTA3/GFUS is at 39 kDa.
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