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Western blot All lanes: TSTA3 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western blot All lanes: TSTA3 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western blot All lanes: TSTA3 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa

TSTA3 Antibody

CSB-PA00935A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGFUS
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSTA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7264
  • Target name
    GFUS
  • Target description
    GDP-L-fucose synthase
  • Target synonyms
    FX, P35B, SDR4E1, TSTA3, GDP-L-fucose synthase, 3-5 epimerase/4-reductase, GDP-4-keto-6-deoxy-D-mannose epimerase-reductase, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, red cell NADP(H)-binding protein, short chain dehydrogenase/reductase family 4E, member 1, testis tissue sperm-binding protein Li 45a, tissue specific transplantation antigen 3, tissue specific transplantation antigen P35B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13630
  • Protein Name
    GDP-L-fucose synthase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TSTA3/GFUS using anti-TSTA3/GFUS antibody (A09921-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSTA3/GFUS antigen affinity purified polyclonal antibody (Catalog # A09921-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSTA3/GFUS at approximately 39 kDa. The expected band size for TSTA3/GFUS is at 39 kDa.
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