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Immunohistochemical staining of human fallopian tube shows weak nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows weak nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows weak nuclear positivity in glandular cells.

Anti-UBA1-25ul

HPA000289
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBA1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22314
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 1
  • Scientific Description
    Rabbit Polyclonal Anti-UBA1 Antibody against Human ubiquitin-like modifier activating enzyme 1. Validated for Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
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