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UBA1 Polyclonal Antibody

CAC15035
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetUBA1
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Overview

  • Supplier
    Biomatik
  • Product Name
    UBA1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7317
  • Target name
    UBA1
  • Target description
    ubiquitin like modifier activating enzyme 1
  • Target synonyms
    A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS, ubiquitin-like modifier-activating enzyme 1, A1S9T and BN75 temperature sensitivity complementing, POC20 centriolar protein homolog, UBA1, ubiquitin-activating enzyme E1 homolog A, testicular secretory protein Li 63
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22314
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 1
  • Scientific Description
    The UBA1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
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