Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E1 Ubiquitin Activating Enzyme Polyclonal Antibody, Unconjugated (bs-6835R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E1 Ubiquitin Activating Enzyme Polyclonal Antibody, Unconjugated (bs-6835R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
E1 Ubiquitin Activating Enzyme Antibody
BS-6835R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetUBA1
Overview
- SupplierBioss Antibodies
- Product NameE1 Ubiquitin Activating Enzyme Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID7317
- Target nameUBA1
- Target descriptionubiquitin like modifier activating enzyme 1
- Target synonymsA1S9; A1S9T; A1S9T and BN75 temperature sensitivity complementing; A1ST; AMCX1; CFAP124; GXP1; POC20; POC20 centriolar protein homolog; SMAX2; testicular secretory protein Li 63; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1X; ubiquitin-like modifier-activating enzyme 1; VEXAS
- HostRabbit
- IsotypeIgG
- Storage Instruction-20°C
- UNSPSC12352203
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