More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-UBE2M Antibody
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Anti-UBE2M Antibody

HPA054551
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBE2M
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBE2M
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9040
  • Target name
    UBE2M
  • Target description
    ubiquitin conjugating enzyme E2 M
  • Target synonyms
    UBC-RS2, UBC12, hUbc12, NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, epididymis secretory sperm binding protein, ubiquitin carrier protein M, ubiquitin conjugating enzyme E2M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M, yeast UBC12 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61081
  • Protein Name
    NEDD8-conjugating enzyme Ubc12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

IHC image of CSB-PA025468OA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
UBE2M AntibodyCSB-PA025468OA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetUBE2M
  • SizePrice
Product group Antibodies
GeneTex
UBE2M antibody [N1C2]GTX101653
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetUBE2M
  • SizePrice
Figure 1. Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody (Catalog # A07478-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-UBC12/UBE2M Antibody Picoband(r)A07478-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUBE2M
  • SizePrice
Product group Antibodies
Bioss
UBE2M Polyclonal AntibodyBS-8382R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetUBE2M
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
UBE2M / UBC12 AntibodyLS-C410542
ApplicationsWestern Blot
TargetUBE2M
  • SizePrice
Atlas Antibodies
PrEST Antigen UBE2MAPREST88489
Protein IDP61081
  • SizePrice
Atlas Antibodies
PrEST Antigen UBE2MAPREST88614
Protein IDP61081
  • SizePrice
Immunohistochemical staining of human rectum shows distinct cytoplasmic and nuclear positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-UBE2M AntibodyHPA057800
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetUBE2M
  • SizePrice