More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/UBE2M / UBC12 Antibody

UBE2M / UBC12 Antibody

LS-C410542
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetUBE2M
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UBE2M / UBC12 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 20kDa, while the observed MW by Western blot was 21kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 20kDa, while the observed MW by Western blot was 21kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9040
  • Target name
    UBE2M
  • Target description
    ubiquitin conjugating enzyme E2 M
  • Target synonyms
    UBC-RS2, UBC12, hUbc12, NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, epididymis secretory sperm binding protein, ubiquitin carrier protein M, ubiquitin conjugating enzyme E2M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M, yeast UBC12 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

IHC image of CSB-PA025468OA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
UBE2M AntibodyCSB-PA025468OA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetUBE2M
  • SizePrice
Product group Antibodies
GeneTex
UBE2M antibody [N1C2]GTX101653
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetUBE2M
  • SizePrice
Figure 1. Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody (Catalog # A07478-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-UBC12/UBE2M Antibody Picoband(r)A07478-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUBE2M
  • SizePrice
Product group Antibodies
Bioss
UBE2M Polyclonal AntibodyBS-8382R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetUBE2M
  • SizePrice
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Product group Antibodies
Atlas Antibodies
Anti-UBE2M AntibodyHPA054551
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetUBE2M
  • SizePrice