More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/UBE2M Polyclonal Antibody

UBE2M Polyclonal Antibody

BS-8382R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetUBE2M
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    UBE2M Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9040
  • Target name
    UBE2M
  • Target description
    ubiquitin conjugating enzyme E2 M
  • Target synonyms
    epididymis secretory sperm binding protein; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC12; UBC-RS2; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Product group Antibodies
Atlas Antibodies
Anti-UBE2M AntibodyHPA054551
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUBE2M
  • SizePrice
Product group Antibodies
GeneTex
UBE2M antibody [N1C2]GTX101653
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetUBE2M
  • SizePrice
IHC image of CSB-PA025468OA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
UBE2M AntibodyCSB-PA025468OA01HU
ApplicationsELISA, ImmunoHistoChemistry
TargetUBE2M
  • SizePrice
Figure 1. Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody (Catalog # A07478-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-UBC12/UBE2M Antibody Picoband(r)A07478-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUBE2M
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
UBE2M / UBC12 AntibodyLS-C410542
ApplicationsWestern Blot
TargetUBE2M
  • SizePrice