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Lane 1: A549 Cells; Probed with WNK1 (7C9) Monoclonal Antibody (bsm-54375R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A549 Cells; Probed with WNK1 (7C9) Monoclonal Antibody (bsm-54375R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A549 Cells; Probed with WNK1 (7C9) Monoclonal Antibody (bsm-54375R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

WNK1 Recombinant Antibody

BSM-54375R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWNK1
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Overview

  • Supplier
    Bioss
  • Product Name
    WNK1 (7C9) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:400-800), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID65125
  • Target name
    WNK1
  • Target description
    WNK lysine deficient protein kinase 1
  • Target synonyms
    HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65, serine/threonine-protein kinase WNK1, erythrocyte 65 kDa protein, prostate-derived sterile 20-like kinase, protein kinase with no lysine 1, protein phosphatase 1, regulatory subunit 167, serine/threonine-protein kinase WNK1 1, serine/threonine-protein kinase WNK1 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4A3
  • Protein Name
    Serine/threonine-protein kinase WNK1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of WNK1 using anti-WNK1 antibody (PB9964). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNK1 antigen affinity purified polyclonal antibody (Catalog # PB9964) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNK1 at approximately 250 kDa. The expected band size for WNK1 is at 251 kDa.
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