Figure 1. Western blot analysis of XBP using anti-XBP antibody (PB9463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: MM231 Whole Cell Lysate at 40ug, Lane 3: MM453 Whole Cell Lysate at 40ug, Lane 4: SKOV Whole Cell Lysate at 40ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP antigen affinity purified polyclonal antibody (Catalog # PB9463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP at approximately 29 kDa. The expected band size for XBP is at 29 kDa.
Figure 1. Western blot analysis of XBP using anti-XBP antibody (PB9463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: MM231 Whole Cell Lysate at 40ug, Lane 3: MM453 Whole Cell Lysate at 40ug, Lane 4: SKOV Whole Cell Lysate at 40ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP antigen affinity purified polyclonal antibody (Catalog # PB9463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP at approximately 29 kDa. The expected band size for XBP is at 29 kDa.
Anti-XBP1 Antibody Picoband(r)
PB9463
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetXBP1
Overview
- SupplierBoster Bio
- Product NameAnti-XBP1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for XBP1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID7494
- Target nameXBP1
- Target descriptionX-box binding protein 1
- Target synonymstax-responsive element-binding protein 5; TREB5; TREB-5; X-box-binding protein 1; XBP-1; XBP2
- HostRabbit
- IsotypeIgG
- Protein IDP17861
- Protein NameX-box-binding protein 1
- Scientific DescriptionBoster Bio Anti-XBP1 Antibody Picoband® catalog # PB9463. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Dysregulated autophagic activity induced in response to chronic intermittent hypoxia contributes to the pathogenesis of NAFLD. Wang D et al., 2022, Front PhysiolRead more
- Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion. Wei W et al., 2013 Jun, Environ ToxicolRead more