More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-ZO1 Antibody
Anti-ZO1 Antibody

Anti-ZO1 Antibody

A121581
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ZO1 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to ZO1.
  • UNSPSC
    12352203

Related products

Product group Antibodies
Bethyl Laboratories
Rabbit anti-TJP1/ZO-1 Antibody, Affinity PurifiedA304-784A
ApplicationsImmunoPrecipitation, Western Blot
TargetTJP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TJP1 / ZO-1 AntibodyLS-C831674
ApplicationsELISA, ImmunoHistoChemistry
TargetTJP1
  • SizePrice
Figure 1. Western blot analysis of TJP1 using anti-TJP1 antibody (PB9234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody (Catalog # PB9234) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 185 kDa.
Product group Antibodies
Boster Bio
Anti-ZO1 tight junction protein/TJP1 Antibody Picoband(r)PB9234-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTJP1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-ZO-1EB09206
ApplicationsImmunoFluorescence, ELISA
TargetTJP1
  • SizePrice
Product group Antibodies
References
GeneTex
ZO-1 antibody [C3], C-termGTX108587
ApplicationsWestern Blot
TargetTJP1
  • SizePrice
Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA001636 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-TJP1 AntibodyHPA001636
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTJP1
  • SizePrice
Product group Antibodies
MedChem Express
ZO-1/TJP1 Antibody (YA3430, Rabbit)HY-P83694
TargetTJP1
  • SizePrice
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
ZO-1/TJP1 Polyclonal AntibodyBS-1329R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTJP1
  • SizePrice
Product group Antibodies
Assay Genie
ZO-1 AntibodyCAB0659
TargetTJP1
  • SizePrice
Product group Antibodies
Biomatik
Tjp1 Polyclonal AntibodyCAC11695
ApplicationsImmunoFluorescence, ELISA
TargetTJP1
  • SizePrice