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ZO1 antibody

70R-13213
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    ZO1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Immunofluorescence staining of U251 cells with CSB-PA023573HA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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TJP1 AntibodyCSB-PA023573HA01HU
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Figure 1. Western blot analysis of TJP1 using anti-TJP1 antibody (PB9234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody (Catalog # PB9234) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 185 kDa.
Product group Antibodies
Boster Bio
Anti-ZO1 tight junction protein/TJP1 Antibody Picoband(r)PB9234-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTJP1
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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA001636 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-TJP1 AntibodyHPA001636
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTJP1
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Product group Antibodies
Bethyl Laboratories
Rabbit anti-TJP1/ZO-1 Antibody, Affinity PurifiedA304-784A
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Product group Antibodies
Antibodies.com
Anti-ZO1 AntibodyA121581
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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