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Tjp1 Polyclonal Antibody

CAC11695
Biomatik
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetTJP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    TJP1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, IF; Recommended dilution: IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7082
  • Target name
    TJP1
  • Target description
    tight junction protein 1
  • Target synonyms
    ZO-1, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07157
  • Protein Name
    Tight junction protein ZO-1
  • Scientific Description
    The Tjp1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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