293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
ZO-1/TJP1 Polyclonal Antibody
BS-1329R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTJP1
Overview
- SupplierBioss
- Product NameZO-1/TJP1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID7082
- Target nameTJP1
- Target descriptiontight junction protein 1
- Target synonymsZO-1, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
- HostRabbit
- IsotypeIgG
- Protein IDQ07157
- Protein NameTight junction protein 1
- Storage Instruction-20°C
- UNSPSC12352203
References
- Tang Y, Wang X, Li J, et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019,13(11):13015-13026. doi: 10.1021/acsnano.9b05679Read this paper
- Zhang W, Yang H, Zhu L, et al. Role of EGFR/ErbB2 and PI(3)K/AKT/e-NOS in Lycium barbarum polysaccharides Ameliorating Endothelial Dysfunction Induced by Oxidative Stress. Am J Chin Med. 2019,47(7):1523-1539. doi: 10.1142/S0192415X19500782Read this paper
- Guo Y, Li H, Liu Z, et al. Impaired intestinal barrier function in a mouse model of hyperuricemia. Mol Med Rep. 2019,20(4):3292-3300. doi: 10.3892/mmr.2019.10586Read this paper
- Xue Y, Huang F, Tang R, et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague-Dawley rats. Food Chem Toxicol. 2019,133:110751. doi: 10.1016/j.fct.2019.110751Read this paper
- Song M, Ye J, Zhang F, et al. Chenodeoxycholic Acid (CDCA) Protects against the Lipopolysaccharide-Induced Impairment of the Intestinal Epithelial Barrier Function via the FXR-MLCK Pathway. J Agric Food Chem. 2019,67(32):8868-8874. doi: 10.1021/acs.jafc.9b03173Read this paper
- Li N, Wang B, Wu Y, et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019,116:109044. doi: 10.1016/j.biopha.2019.109044Read this paper
- Li T , Gao J , Du M , et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019,10(6):3368-3378. doi: 10.1039/c8fo01967cRead this paper
- Xia W, Zhang H, Pan Z, et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019,72:211-217. doi: 10.1016/j.intimp.2019.04.009Read this paper
- Li X, Wang H, Zhang Y, et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019,7:e6715. doi: 10.7717/peerj.6715Read this paper
- Chen M , Liu Y , Xiong S , et al. Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model. Food Funct. 2019,10(5):2390-2398. doi: 10.1039/c9fo00123aRead this paper
Datasheet
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