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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need a specific antibody and can’t find it in our webshop, please contact our technical support.

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A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H4K8ac (cat. No. bs-53133R) with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000.
Product group Antibodies
Bioss Antibodies
H4K8ac Polyclonal AntibodyBS-53133R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetH4C9
  • SizePrice
Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 μg) from control (lane 1) and from transfected cells (lane 2) were analyzed by Western blot using PML Polyclonal Antibody (Cat. No. bs-53134R). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The smaller band of approximately 60 kDa might represent the endogenous PML protein.
Product group Antibodies
Bioss Antibodies
PML Polyclonal AntibodyBS-53134R
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPML
  • SizePrice
A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3S10p (Cat. No. bs-53135R) with peptides containing other modifications of histone H3 or the unmodified H3S10 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest. Note that the antibody does not recognize the H3S10p modification if the neighboring K9 is acetylated or trimethylated.
Product group Antibodies
Bioss Antibodies
H3S10p Polyclonal AntibodyBS-53135R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Nuclear extracts from HeLa cells (40 μg) were analyzed by Western blot using the Bioss antibody against JMJD2a (Cat. No. bs-53136R) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
JMJD2a Polyclonal AntibodyBS-53136R
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetKDM4A
  • SizePrice
HeLa cells were stained with the Bioss antibody against H3K18ac (cat. No. bs-53137R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Product group Antibodies
Bioss Antibodies
H3K18ac Polyclonal AntibodyBS-53137R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
Western blot was performed on whole cell extracts (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4pan Polyclonal Antibody(bs-53138R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
H4pan Polyclonal AntibodyBS-53138R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH4C9
  • SizePrice
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H2Apan Polyclonal Antibody (bs-53140R). The antibody was diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
H2Apan Polyclonal AntibodyBS-53140R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH2AW
  • SizePrice
Product group Antibodies
Bioss Antibodies
H3R17me2(asym)K18ac Polyclonal AntibodyBS-53141R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
Product group Antibodies
Bioss Antibodies
H3K27ac Polyclonal AntibodyBS-53142R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Western blot was performed on histone extracts (30 μg) from HeLa cells using H3K64me3 Polyclonal Antibody (bs-53144R). The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.
Product group Antibodies
Bioss Antibodies
H3K64me3 Polyclonal AntibodyBS-53144R
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
To test the cross reactivity of the H3K56ac Polyclonal Antibody (bs-53145R), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K56. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
Product group Antibodies
Bioss Antibodies
H3K56ac Polyclonal AntibodyBS-53145R
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H2AK5ac Polyclonal Antibody (bs-53146R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
H2AK5ac Polyclonal AntibodyBS-53146R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH2AW
  • SizePrice
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