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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

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A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3K36me3 (bs-53116R) with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityDrosophila, Human, Zebra Fish, Other Species
TargetH3C1
  • SizePrice
Histone extracts (15 μg) from HeLa cells were analyzed by Western blot using the Bioss antibody against H3K9me2 (Cat. No. bs-53118R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Plant, Other Species
TargetH3C1
  • SizePrice
Histone extracts (15 μg) from HeLa cells were analyzed by Western blot using the Bioss antibody against H3K9me1 (Cat. No. bs-53120R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, Western Blot, ELISA
ReactivityHuman, Other Species
TargetH3C1
  • SizePrice
Human osteosarcoma (U2OS) cells were stained with the Bioss antibody against H3K79me3 (Cat. No. bs-53121R) and with DAPI. Cells were fixed with 2.5% formaldehyde for 30’ and blocked with PBS/TX-100 containing 1% BSA. Figure A: cells were immunofluorescently labeled with the H3K79me3 antibody (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right). Figure B and C: staining of the cells with the H3K79me3 antibody after incubation of the antibody with 2 ng/μl H4K20me3 and H3K79me3 peptide, respectively.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Yeast
TargetH3C1
  • SizePrice
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H3K27me3 (Cat. No. bs-53122R) diluted 1:500 in TBS-Tween containing 5% skimmed milk.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityDrosophila, Human, Mouse, Plant, Rat, Schistosoma japonicum, Zebra Fish, Other Species
TargetH3C1
  • SizePrice
HeLa cells were stained with the Bioss antibody against HP1α (Cat. No. bs-53123R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetCBX5
  • SizePrice
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with Med26 Polyclonal Antibody (bs-53124R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation.
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetMED26
  • SizePrice
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 3) using H3K79me1 Polyclonal Antibody (Cat. No. bs-53126R). The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Yeast
TargetH3C1
  • SizePrice
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using p53 Polyclonal Antibody (bs-53127R) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. Followed by secondary antibody incubation.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Bioss antibody directed against human DNMT3A (Cat. No. bs-53128R), crude serum and Flow Through, in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:500.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetDNMT3A
  • SizePrice
U2OS cells were stained with the Bioss antibody against H3K36me1 (cat. No. bs-53131R) and with DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. Figure B, C, D, and E: staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36, respectively.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Western blot was performed on histone extracts (15 μg, lane 1) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 2) using H3K27me3S28p Polyclonal Antibody (Cat. No. bs-53132R) diluted 1:100 in TBS-Tween containing 5% skimmed milk.
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice