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Western blot analysis of various lysates using AP2M1 Rabbit pAb (TA373561) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Western blot analysis of various lysates using AP2M1 Rabbit pAb (TA373561) at 1:500 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 10s.
Western blot analysis of various lysates using AP2M1 Rabbit pAb (TA373561) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

AP2M1 Rabbit Polyclonal Antibody

TA373561
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAP2M1
Price on request
100 ul
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More than 130 suppliers
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Overview

  • Supplier
    OriGene
  • Product Name
    AP2M1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1173
  • Target name
    AP2M1
  • Target description
    adaptor related protein complex 2 subunit mu 1
  • Target synonyms
    AP50, CLAPM1, MRD60, mu2, AP-2 complex subunit mu, AP-2 mu 2 chain, HA2 50 kDA subunit, adaptin-mu2, adaptor protein complex AP-2 subunit mu, adaptor related protein complex 2 mu 1 subunit, adaptor-related protein complex 2 subunit mu, clathrin adaptor complex AP2, mu subunit, clathrin assembly protein complex 2 medium chain, clathrin assembly protein complex 2 mu medium chain, clathrin coat adaptor protein AP50, clathrin coat assembly protein AP50, clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CW1
  • Protein Name
    AP-2 complex subunit mu
  • Scientific Description
    AP2M1 Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50 kDa. The expected band size for AP2M1 is at 50 kDa.
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