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Western Blot analysis of HuvEc cells using API5 Polyclonal Antibody
Western Blot analysis of HuvEc cells using API5 Polyclonal Antibody
Western Blot analysis of HuvEc cells using API5 Polyclonal Antibody

API5 Antibody

CSB-PA000902
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetAPI5
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Overview

  • Supplier
    Cusabio
  • Product Name
    API5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8539
  • Target name
    API5
  • Target description
    apoptosis inhibitor 5
  • Target synonyms
    AAC-11, AAC11, apoptosis inhibitor 5, FIF, antiapoptosis clone 11 protein, cell migration-inducing gene 8 protein, fibroblast growth factor 2-interacting factor 2, migration-inducing protein MIG8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZZ5
  • Protein Name
    Apoptosis inhibitor 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of API5 using anti-API5 antibody (PA1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Testis Tissue Lysate, Lane 4: Rat Placenta Tissue Lysate, Lane 5: MCF-7 Cell Lysate, Lane 6: HELA Cell Lysate, Lane 7: CEM Cell Lysate, Lane 8: SMMC Cell Lysate, Lane 9: COLO320 Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-API5 antigen affinity purified polyclonal antibody (Catalog # PA1009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for API5 at approximately 59 kDa. The expected band size for API5 is at 59 kDa.
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