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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, DAB staining.

API5 Polyclonal Antibody

BS-1252R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetAPI5
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Overview

  • Supplier
    Bioss
  • Product Name
    API5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8539
  • Target name
    API5
  • Target description
    apoptosis inhibitor 5
  • Target synonyms
    AAC-11, AAC11, apoptosis inhibitor 5, FIF, antiapoptosis clone 11 protein, cell migration-inducing gene 8 protein, fibroblast growth factor 2-interacting factor 2, migration-inducing protein MIG8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZZ5
  • Protein Name
    Apoptosis inhibitor 5
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of API5 using anti-API5 antibody (PA1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Testis Tissue Lysate, Lane 4: Rat Placenta Tissue Lysate, Lane 5: MCF-7 Cell Lysate, Lane 6: HELA Cell Lysate, Lane 7: CEM Cell Lysate, Lane 8: SMMC Cell Lysate, Lane 9: COLO320 Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-API5 antigen affinity purified polyclonal antibody (Catalog # PA1009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for API5 at approximately 59 kDa. The expected band size for API5 is at 59 kDa.
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