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Western Blot analysis of Human brain malignant glioma tissue, A172 and 293T cell using ARHGEF7 Polyclonal Antibody at dilution of 1:800
Western Blot analysis of Human brain malignant glioma tissue, A172 and 293T cell using ARHGEF7 Polyclonal Antibody at dilution of 1:800
Western Blot analysis of Human brain malignant glioma tissue, A172 and 293T cell using ARHGEF7 Polyclonal Antibody at dilution of 1:800

ARHGEF7 Polyclonal Antibody

E-AB-14609
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ARHGEF7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.2mg/ml
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGEF7 using anti-ARHGEF7 antibody (A02764-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse skeletal muscle lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF7 antigen affinity purified polyclonal antibody (Catalog # A02764-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF7 at approximately 80 kDa. The expected band size for ARHGEF7 is at 90 kDa.
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