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ProductsBack/ATF4 Antibody: FITC

ATF4 Antibody: FITC

ORB150634
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetATF4
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Overview

  • Supplier
    Biorbyt
  • Product Name
    ATF4 Antibody: FITC
  • Delivery Days Customer
    16
  • Application Supplier Note
    1 microg/ml of SMC-447 was sufficient for detection of ATF4 in 20 microg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB (1:1000) ICC, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N360A/24 (Formerly sold as S360A-24)
  • Concentration
    1 mg/ml
  • Conjugate
    FITC
  • Gene ID468
  • Target name
    ATF4
  • Target description
    activating transcription factor 4
  • Target synonyms
    CREB-2, CREB2, TAXREB67, TXREB, cyclic AMP-dependent transcription factor ATF-4, DNA-binding protein TAXREB67, cAMP response element-binding protein 2, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, cyclic AMP-responsive element-binding protein 2, tax-responsive enhancer element B67, tax-responsive enhancer element-binding protein 67
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP18848
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-4
  • Scientific Description
    Mouse monoclonal antibody against ATF4 conjugated to FITC
  • Reactivity
    Human, Rat
  • Storage Instruction
    See Manual
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.
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