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ATP5A Recombinant Antibody, Biotin Conjugated

BSM-61444R-BIOTIN
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATP5F1A
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Overview

  • Supplier
    Bioss
  • Product Name
    ATP5A Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID498
  • Target name
    ATP5F1A
  • Target description
    ATP synthase F1 subunit alpha
  • Target synonyms
    ATP5A, ATP5A1, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MC5DN4A, MC5DN4B, MOM2, OMR, ORM, hATP1, ATP synthase F(1) complex subunit alpha, mitochondrial, ATP synthase alpha chain, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP sythase (F1-ATPase) alpha subunit, epididymis secretory sperm binding protein Li 123m, mitochondrial ATP synthetase, oligomycin-resistant
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25705
  • Protein Name
    ATP synthase F(1) complex subunit alpha, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody (Catalog # A32267-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 54 kDa. The expected band size for ATP5F1A is at 54 kDa.
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