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Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody

ATP5A1 Antibody

CSB-PA000951
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATP5F1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP5A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID498
  • Target name
    ATP5F1A
  • Target description
    ATP synthase F1 subunit alpha
  • Target synonyms
    ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5A1; ATP5AL2; ATPM; COXPD22; epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; mitochondrial ATP synthetase, oligomycin-resistant; MOM2; OMR; ORM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25705
  • Protein Name
    ATP synthase subunit alpha, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody HPA040622 (A) shows similar protein distribution across tissues to independent antibody HPA044202 (B).
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Figure 1. Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody (Catalog # A32267-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 54 kDa. The expected band size for ATP5F1A is at 54 kDa.
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