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Goat anti-ATP5A1

EB12389
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP5F1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATP5A1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID498
  • Target name
    ATP5F1A
  • Target description
    ATP synthase F1 subunit alpha
  • Target synonyms
    ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5A1; ATP5AL2; ATPM; COXPD22; epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; mitochondrial ATP synthetase, oligomycin-resistant; MOM2; OMR; ORM
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004037.1; NP_001244263.1; NP_001001935.1. GeneIDs all Nonhuman: 11946 (mouse); 65262 (rat);. Purification: Antigen affinity purified. Names and symbols: ATP5A1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP5A; ATP5AL2; ATPM; MOM2; OMR; ORM; hATP1; ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transportin
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody (Catalog # A32267-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 54 kDa. The expected band size for ATP5F1A is at 54 kDa.
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